New vaccine strategy with chimeric virus particles without adjuvant

Researchers of the CReSA, in collaboration with researchers of the Centro de Investigación en Sanidad Animal (CISA-INIA) and Centro Nacional de Biotecnología (CNB-CSIC) have been able to obtain a protective anti-viral cytotoxic response using chimeric calicivirus-like particles without adjuvant.

The results of this study show the generation of chimeric pseudo-particles from the capsid protein of rabbit hemorrhagic disease virus. Mice immunized with the chimeric viral pseudo-particles without adjuvant were able to induce specific cellular responses mediated by cytotoxic and memory T cells. More importantly, immunization with chimeric virus-like particles was able to resolve an infection by a recombinant vaccinia virus.

These data were exposed as an oral communication (Elisa Crisci, PhD student of the CReSA, II Congress of the Catalan Society for Immunology, Barcelona 20-21st November, 2008). Results will be exposed soon as a paper in the journal Virology.

The abstract of the paper is shown next:

 CHIMERIC CALICIVIRUS-LIKE PARTICLES ELICIT PROTECTIVE ANTI-VIRAL CYTOTOXIC RESPONSES WITHOUT ADJUVANT
E. Crisci1, H. Almanza2, I. Mena2, L. Córdoba1, E. Gómez-Casado3, J.R. Castón4, L. Fraile1, J. Bárcena2, M. Montoya1*
1Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. 2Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, Madrid, Spain. 3Dpt. of Biotecnología, INIA, Autopista A-6, Km 7, 28040-Madrid, Spain. 4Dpt. of Estructura de Macromoléculas, Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049, Madrid, Spain

*Corresponding author: Dr. Maria Montoya. Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autonoma de Barcelona, Barcelona, 08193, Spain Tel: +34-93-581-4562 Fax: +34-93-581-4490 E-mail: maria.montoya@cresa.uab.es

We have analyzed the potential of virus-like particles (VLPs) from rabbit hemorrhagic disease virus (RHDV) as a delivery system for foreign T-cell epitopes. To accomplish this goal, we generated chimeric RHDV VLPs incorporating a CD8+ T-cell epitope (SIINFEKL) derived from chicken ovalbumin (OVA). The OVA epitope was inserted in the capsid protein (VP60) of RHDV at two different locations: 1) the N-terminus, predicted to be facing to the inner core of the VLPs, and 2) a novel insertion site predicted to be located within an exposed loop. Both constructions correctly assembled into VLPs. In vitro,the chimeric VLPs activated dendritic cells for TNF-alpha secretion and they were processed and presented to specific T cells. In vivo, mice immunized with the chimeric VLPs without adjuvant were able to induce specific cellular responses mediated by cytotoxic and memory T cells. More importantly, immunization with chimeric VLPs was able to resolve an infection by a recombinant vaccinia virus expressing OVA protein.

To contact with the author of this paper:

Elisa Crisci
PhD student
Immunology Unit (CReSA)
Email: elisa.crisci@cresa.uab.cat
Telephone no.: +34 93.581.45.27
Fax: +34 93 581 44 90
Edifici CReSA. Campus UAB
08193 Bellaterra (Barcelona) España


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