Doctoral thesis on Classical Swine Fever

On January 25, 2012, Joan Tarradas Font (PhD student of the CReSA) will defend his doctoral thesis entitled “Studies on immunopathogenesis of classical swine fever virus for developing of new vaccination methods”, directed by Dr Llilianne Ganges.

The event will be held on January 25, 2012 (12:00h), in the Sala d’Actes of the Facultat de Veterinària (Universitat Autònoma de Barcelona).

The work of this thesis helped to clarify the role of cellular responses associated with IFN-gamma pathway in protection against classical swine fever virus (CSFV). On the other hand, it demonstrated the involvement of alpha IFN in the development of the acute form of the disease. High levels of alpha IFN detected in the sera of infected animals may be related to the immunosuppression, survival of virus in the host and, at the same time, can be considered as a marker of viral replication.

We characterized the immunomodulatory capacity of the swine chemokine CCL20 on DNA vaccination against CSFV. The co-injection of plasmid expressing this chemokine with the E2 protein can induce a sterilizing protection against the virus.

Finally, this thesis showed that the strategy of vaccination with dendrimeric peptide can be used as a DIVA (Differentiation of Infected and Vaccinated Animals) vaccine candidate against the virus. In addition, it has been shown that dendrimers are an effective tool for the characterization of new B and T CSFV epitopes in vivo.

Download the articles derived from this thesis:

Immunomodulatory effect of swine CCL20 chemokine in DNA vaccination against CSFV. Tarradas J, Álvarez B, Fraile L, Rosell R, Muñoz M, Galindo-Cardiel I, Domingo M, Dominguez J, Ezquerra A, Sobrino F, Ganges L. Vet Immunol Immunopathol. 2011 Aug 15;142(3-4):243-51.

Link: http://www.ncbi.nlm.nih.gov/pubmed/21684019

Partial protection against classical swine fever virus elicited by dendrimeric vaccine-candidate peptides in domestic pigs. Tarradas J, Monsó M, Muñoz M, Rosell R, Fraile L, Frías MT, Domingo M, Andreu D, Sobrino F, Ganges L. Vaccine. 2011 Jun 10;29(26):4422-9.

Link: http://www.ncbi.nlm.nih.gov/pubmed/21496472

Interferon-gamma induction correlates with protection by DNA vaccine expressing E2 glycoprotein against classical swine fever virus infection in domestic pigs.Tarradas J, Argilaguet JM, Rosell R, Nofrarías M, Crisci E, Córdoba L, Pérez-Martín E, Díaz I, Rodríguez F, Domingo M, Montoya M, Ganges L. Vet Microbiol. 2010 Apr 21;142(1-2):51-8.

Link: http://www.ncbi.nlm.nih.gov/pubmed/19896784

To contact the director of this thesis:

Llilianne Ganges Espinosa
Principal investigator of the research Pathogenesis and profilaxis of Pestivirus infections
Email: llilianne.ganges@cresa.uab.cat
Phone no.: +34 935814620
Fax: +34 93581490
08193 Bellaterra (Barcelona) Spain

¡Recommend this page!: