Swine influenza virus interacts with porcine dendritic cells

Scientists from CReSA studied the particular interaction of swine influenza virus with porcine dendritic cells. This virus causes sub-acute or acute respiratory infections on swine farms and pigs can act as “mixing vessels” for new influenza strains.

Dendritic cells (DCs) mediate the induction of immunity to pathogens. The interaction of a swine influenza virus (SwIV) field strain isolated in a Catalonian farm (H3N2) with porcine DC and the ability of infected porcine DC to transmit the infection to permissive cells were evaluated by CReSA researchers.

For this study, porcine bone marrow derived DCs (poBMDCs) were exposed to a circulating strain of H3N2 SwIV in vitro. Infection of poBMDCs resulted in structures resembling influenza virus inside poBMDCs in vesicles and also free in cytoplasm. Viral progeny was undetectable in supernatant but limited replication was detected in the first 8 h after infection. However, viral particles from infected-poBMDCs were able to induce cytopathic effect in susceptible cells only when cell-to-cell interaction was favoured.

The data generated in our studies reveal the particular interaction of H3N2 SwIV with conventional porcine DCs. This interaction may be determinant at the time of priming an effective immune response by DC.

Dendritic cell infected with H3N2 SwIV. Next to the Golgi complex, several H3N2 SwIV like-particles were observed (arrows). Bar = 200 nm. Photograph taken by transmission electronic microscopy.

This work has been published in: Tufária Mussá, Carolina Rodriguez-Cariño, Myriam Pujol, Lorena Córdoba, Núria Busquets, Elisa Crisci, Javier Dominguez, Lorenzo Fraile, María Montoya. Interaction of porcine conventional dendritic cells with swine influenza virus, Virology 420 (2011) 125–134.

To contact the author of this paper:

Tufària Mussá
PhD student
Email: tufaria.mussa@cresa.uab.cat
Phone no.: +34 93 581 45 61
Fax: +34 93 581 44 90
Edifici CReSA. Campus UAB
08193 Bellaterra (Barcelona) Spain

Download the full paper: http://www.sciencedirect.com/

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