Virus-like particles are suitable as vaccine vectors against swine viruses

Researchers of the CReSA have demonstrated the strong potential and immunogenicity of virus-like particles in pig and, therefore, their suitability as appealing vaccine vectors for veterinary viral vaccinology. This is the first immunological report on the potential use of chimeric RHDV-VLPs as antigen carriers in pigs.

Virus-like particles (VLPs) have received considerable attention due to their potential application in veterinary vaccines and, in particular, VLPs from rabbit haemorrhagic disease virus (RHDV) have successfully shown to be good platforms in mice for inducing immune responses against an inserted foreign epitope.

The aim of the study carried out by researchers of the CReSA was to assess the immunogenicity of chimeric RHDV-VLPs as vaccine vectors in pigs. For this purpose, pigs were inoculated twice in a two-week interval with chimeric RHDV-VLPs at different doses intranasally or intramuscularly. One intramuscularly treated group was also inoculated with adjuvant at the same time. Results are shown above:

  • Specific IgG and IgA antibodies against RHDV-VLPs were induced and such levels were higher in the adjuvanted group compared with other groups.
  • Interestingly, IgA responses were higher in groups inoculated intramuscularly than those that received the VLPs intranasally.
  • Two weeks after the last immunisation, specific IFN-γ-secreting cells were detected by ELISPOT.
  • The adjuvanted group exhibited the highest IFN-γ-secreting cell numbers and lymphoproliferative specific T cell responses.

Model de pseudopartícula viral o virus-like particle (VLP). Figura creada per Carla Martínez Castro y Elisa Crisci

Model of virus-like particle (VLP).
Figure created by Carla Martínez Castro and Elisa Crisci.

This work has been recently published in: Crisci E, Fraile L, Moreno N, Blanco E, Cabezón R, Costa C, Mussá T, Baratelli M, Martinez-Orellana P, Ganges L, Martínez J, Bárcena J, Montoya M. Chimeric calicivirus-like particles elicit specific immune responses in pigs. Vaccine. 2012 Mar 23;30(14):2427-39.

To contact the author of this paper:

Elisa Crisci
Researcher
Email: elisa.crisci@cresa.uab.cat
Telephone no.: +34 93 581 45 61
Fax: +34 93 581 44 90
Edifici CReSA. Campus UAB
08193 Bellaterra (Barcelona) Spain

Download the full paper:

http://www.sciencedirect.com/science/article/pii/S0264410X12001077

 
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