Following classical swine fever

The research trajectory of the CReSA in classical swine fever during the last 4 years has resulted in new insights in the protective response against the virus, and in the viral evolution in endemics areas. This information is the great interest for the development of new diagnostic techniques and new vaccines. These studies have resulted in the publication of six articles in prestigious international journals.

Classical Swine Fever (CSF) is a highly contagious viral disease that affects domestic and wild pigs. This is an endemic disease in some countries of Central America and the Caribbean, South America, Southeast Asia and Eastern Europe. The European Union is considered high risk area of re-emergence of the disease due to the high density in the pig population, the non-vaccination policy currently followed and its proximity to Eastern European countries.

The CReSA research on CSF virus (CSFV) is focused on studies of viral immunopathogenesis and viral evolution to develop new diagnostic methods and vaccination strategies.

Dr. Llilianne Ganges, investigator of the CReSA on CSFV, summarizes the main results obtained during these 4 years: "We have conducted studies of positive selection pressure of CSFV in an endemic area subject to control by vaccination with the live attenuated vaccine. The results suggest a possible association between viral escape variants and the observed alterations in virulence and pathogenesis of the virus after 20 years of circulation".

In addition, Dr. Ganges says: "We have developed new recombinant vaccines based on dendrimeric peptides, new molecular adjuvants and molecular diagnostic techniques for the rapid detection and quantification of viral nucleic acid. Likewise, others studies developed during the project demonstrated the ability of the E2 glycoprotein of inducing a rapid and strong interferon gamma response that correlates with protection against the CSFV before the induction of neutralizing antibodies. "

Moreover, she points out: “The studies carried out have shown the ability of virulent strains to induce a strong response of the type I interferon that correlates with high viral replication in pigs at very short times post infection. Finally, we have identified new viral epitopes involved in protection and that can be of great interest to develop new techniques for serological diagnosis more effective than the existing ones”.

Dr. Ganges leads the CReSA research group on Pestivirus formed further by Rosa Rosell, Mariano Domingo, Francesc Xavier Abad and Marta Muñoz.

This project was funded by the Spanish Ministry for Science and Innovation (New vaccine strategies against classical swine fever virus. Study of mechanisms involved in viral immunopathogenesis (BIO2008-04487-C03-03). While developing the project, we have collaborated with other institutions such as the University of Lleida (UDL), the Center of Molecular Biology Severo Ochoa (CBMSO, in Madrid), the Universitat Pompeu Fabra (UPF, Barcelona) and the National Center of Agricultural and animal Health (CENSA, in La Habana, Cuba).

To contact with the responsible in charge of this research line:

Dr Llilianne Ganges Espinosa
Researcher in charge of the research line “Pathogenesis and profilaxis of Pestivirus infections”
Edifici CReSA. Campus UAB
08193 Bellaterra (Barcelona) Spain
Phone no.: (+34) 935814620
Fax: (+34) 935814490

To consult the articles resulting from the project:

Interferon-gamma induction correlates with protection by DNA vaccine expressing E2 glycoprotein against classical swine fever virus infection in domestic pigs. Tarradas J, Argilaguet JM, Rosell R, Nofrarías M, Crisci E, Córdoba L, Pérez-Martín E, Díaz I, Rodríguez F, Domingo M, Montoya M, Ganges L. Vet Microbiol. 2010 Apr 21;142(1-2):51-8.

Immunomodulatory effect of swine CCL20 chemokine in DNA vaccination against CSFV. Tarradas J, Álvarez B, Fraile L, Rosell R, Muñoz M, Galindo-Cardiel I, Domingo M, Dominguez J, Ezquerra A, Sobrino F, Ganges L. Vet Immunol Immunopathol. 2011 Aug 15;142(3-4):243-51.

Development and validation of a novel SYBR Green real-time RT-PCR assay for the detection of classical swine fever virus evaluated on different real-time PCR platforms. Pérez LJ, Díaz de Arce H, Tarradas J, Rosell R, Perera CL, Muñoz M, Frías MT, Nuñez JI, Ganges L. J Virol Methods. 2011 Jun;174(1-2):53-9.

Partial protection against classical swine fever virus elicited by dendrimeric vaccine-candidate peptides in domestic pigs. Tarradas J, Monsó M, Muñoz M, Rosell R, Fraile L, Frías MT, Domingo M, Andreu D, Sobrino F, Ganges L. Vaccine. 2011 Jun 10;29(26):4422-9.

Positive selection pressure on the B/C domains of the E2-gene of classical swine fever virus in endemic areas under C-strain vaccination.
Pérez LJ, Díaz de Arce H, Perera CL, Rosell R, Frías MT, Percedo MI, Tarradas J, Dominguez P, Núñez JI, Ganges L. Infect Genet Evol. 2012, 7: 1405-12.

A T-cell epitope on NS3 non-structural protein enhances the B and T cell responses elicited by dendrimeric constructions against CSFV in domestic pigs
.Tarradas J, Monsó M, Fraile L, de la Torre BG, Muñoz M, Rosell R, Riquelme C, Pérez LJ, Nofrarías M, Domingo M, Sobrino F, Andreu D, Ganges L. Vet Immunol Immunopathol. 2012 Aug 17. [Epub ahead of print].

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